her-2 (a0485, polyclonal rabbit antibody  (Agilent technologies)

Journal: Journal of Clinical and Diagnostic Research : JCDR

Article Title: HER 2 Expression in Gastric and Gastro-esophageal Junction (GEJ) Adenocarcinomas

doi: 10.7860/JCDR/2015/12581.5630

Figure Lengend Snippet: [Table/Fig-7]:

Article Snippet: Immnohistochemistry was done using Rabbit polyclonal HER 2 antibody in the dilution of 1:600 using Dako REALTM En Vision DetectionTM system, (Peroxidase /DAB+, K5007).

Techniques:

Journal: International Journal of Oncology

Article Title: A model of spontaneous mouse mammary tumor for human estrogen receptor- and progesterone receptor-negative breast cancer

doi: 10.3892/ijo.2014.2657

Figure Lengend Snippet: Immunohistochemical evaluation of ER-α, PR, HER-2/neu in mouse and human female DCIS. Strong and diffuse nuclear expression of (A1) ER-α, (B1) PR in human mammary cancer, but the model mouse cancer cells with no ER-α (A2 and A3), PR (B2 and B3) expression. Human mammary tumor with strong membraneous stain (3+) of human epidermal growth factor receptor 2 (HER2) (C1). Mouse cancer cell showing weak membrane (1+) HER-2/ neu expression (C2 and C3). Immunoperoxidase-DAB. Bar, 10 μm.

Article Snippet: The membrane was then incubated with the cyclin D1, VEGF, c-Myc HER-2/ neu polyclonal rabbit anti-human antibody at 1:1,000 dilution (DakoCytomation) in PBS-T plus 2% skim milk for 2 h, washed five times with PBS-T, and incubated for 1 h with peroxidase-conjugated goat anti-rabbit secondary antibodies (Sigma-Aldrich) in PBS-T plus 2% milk.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: International Journal of Oncology

Article Title: A model of spontaneous mouse mammary tumor for human estrogen receptor- and progesterone receptor-negative breast cancer

doi: 10.3892/ijo.2014.2657

Figure Lengend Snippet: Intensity of bands indicates the expression level of the corresponding molecular markers by western immunoblot with the Santa Cytomation anti-Myc, -VEGF, -cyclin D1 antibody in 6 Kunming mouse mammary tumors. (A) c-Myc expression is weaker in normal mammary gland (Con), moderate to higher expression in 2 to 4 weeks than normal, but are the strongest in 6–8-week Kunming mouse mammary tumors. (B) VEGF band is weaker in Con, but at 2–8 weeks are gradually stronger in mammary tissues. (C) Cyclin D1 expression is weaker in Con while it is the strongest at 6 weeks in mammary tissues, moderate to higher expression at 2, 4, 8 weeks compared to normal.

Article Snippet: The membrane was then incubated with the cyclin D1, VEGF, c-Myc HER-2/ neu polyclonal rabbit anti-human antibody at 1:1,000 dilution (DakoCytomation) in PBS-T plus 2% skim milk for 2 h, washed five times with PBS-T, and incubated for 1 h with peroxidase-conjugated goat anti-rabbit secondary antibodies (Sigma-Aldrich) in PBS-T plus 2% milk.

Techniques: Expressing, Western Blot